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低温诱导植物细胞染色体数目变化 - 虚拟实验
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🧬 低温诱导植物细胞染色体数目变化

虚拟实验 - 探索染色体加倍的奥秘

📋 实验步骤

1

培养根尖

将蒜放入培养皿

2

低温处理

4℃冷藏48-72小时

3

剪取根尖

取0.5-1cm根尖

4

固定染色

卡诺氏液+染色

5

显微观察

对比染色体数目

👉 请将大蒜拖入培养皿开始实验

🔬 实验操作台

培养皿

将大蒜拖到这里

大蒜

💡 关键知识点

低温作用机制

低温抑制纺锤体形成,染色体无法分离到两极,导致染色体数目加倍

染色体加倍

二倍体(2n)→ 四倍体(4n),细胞染色体数目翻倍

实验温度

4℃冷藏48-72小时,温度过高或过低都会影响实验效果

材料选择

选择根尖分生区细胞,该区域细胞分裂旺盛,易于观察

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